LIFE CYCLE OF CULTURED MUSHROOM – STAGE 2

Inoculation

For germination, the tissue is spread on PDA in a container under controlled and sterile conditions. From the tissue cultured, the strongest strains of mycelium are craft by selection to increase the harvest.

Then the selected mycelium strains are transferred on grain to “spawn” the mycelium and to increase mycelium mass. After the grain has been completely colonized by the mushroom, it can be used to inoculate the substrate. Once the mycelium has grown through the substrate, fruiting is initiated under control environmental conditions (humidity, temperature, fresh air supply).

Mushroom spawn – Grain

Many different types of grains can be used for spawn- including sorghum, soya, red and green beans, and even popcorn. The most common grain used for mushroom cultivation is Sorghum.

The 2 bottles below are GREEN beans.

How many spawn grains you will require depend on the weight of each mushroom bag and also how many mushroom bags you want to produce.

Rule of Thumb: 2-3g for 700-800g of substrate.

ONLINE COURSES

STEP BY STEP GUIDE ON HOW TO PREPARE POTATO DEXTROSE AGAR

STEP BY STEP GUIDE ON HOW TO PERFORM TISSUE CULTURE

STEP BY STEP GUIDE ON HOW TO PREPARE GRAINS

STEP BY STEP GUIDE ON HOW TO PREPARE GRAINS FOR INOCULATION

 

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